• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The invention relates to a screening device (1) for separating an egg cell-cumulus complex from a punctate. According to the invention, the screening device has a flat, punctate-impermeable shell (2) which is provided with a cylindrical or conically widening wall region (5) adjoining the edge (4) of the flat shell (2) and comprising at least one screen surface (5). 6), which is suitable for separating the egg cell cumulus complex from the punctate, and a filling edge (7) bounding, preferably annular retaining edge (8).
    公开号:AT519299A1
    申请号:T51002/2016
    申请日:2016-11-03
    公开日:2018-05-15
    发明作者:Hans-Peter Steiner Dr
    申请人:Dr Hans Peter Steiner；
    IPC主号:
    专利说明:

    The invention relates to a screening device for separating an egg cell-cumulus complex from a punctate and a container for use of such a screening device.
    An important step in IVF (In Vitro Fertilization) is the follicle puncture of the ovaries to obtain eggs. Ultrasound-guided transvaginal ejection of the follicle and puncture in a collection tube (for example, a NUNC® tube) collected. Once the tube is filled with about 8 cc of punctate, it is changed to an empty tube. The full tube is given to the embryologist, who empties the contents of the tube into a Petri dish and examines under the microscope for the presence of the cumulus complex surrounding the egg. Internationally common is the English abbreviation OCC (Oocyte Cumulus Complex).
    A puncture set for ultrasound-guided transvaginal puncture of follicles is previously known, for example, from EP 1 967 147 B1, in which a thinner, single-lumen puncture area is formed following the needle tip, on which, starting from a connection point in which a rinsing tube is attached in a liquid-tight manner to the puncture cannula, a double-lumen region adjoins, in which the puncturing cannula runs in the flushing line, so that between the outer wall of the puncture cannula and the inner wall of the flushing line, a flushing channel is formed, which has at its distal end at least one crossing opening in the suction channel of the puncturing cannula. The suction channel is fed via a suction line into a collecting tube for the egg cell-cumulus complex, wherein the suction line and a leading to a vacuum source connecting line are arranged in a insertable into the collection tube stopper.
    The existing blood admixture of the puncture usually requires a portionwise emptying of the collecting tube (usually 2 to 3 times per tube). If this is not done, the detection of the egg-cumulus complex under the microscope is difficult. Previously, half of the collection tube filled with about 8 cm3 had to be emptied halfway into a Petri dish, and the mostly bloody punctate should be examined for the presence of the egg cell-cumulus complex (OCC). If you have found the OCC, it is absorbed in a pipette and another in a Petri
    Small bowl transferred for further examination. The residual liquid is discarded. This process of fractionation thus had to be treated by treatment with e.g. ten full collection tubes at least 20 to 30 times.
    So far, only in isolated cases, the puncture was sent through a cell strainer to separate the liquid components. The reason for this is the fact that in conventional cell sieves the OCC adversely adheres to the bottom of the screen and is difficult to detect with the pipette.
    Such a cell strainer, which is inserted at the upper end of a collecting tube and serves to remove impurities, such as supporting tissue, etc., from a suspension containing lymphocytes, is known, for example, from EP 0 593 767 B1.
    The object of the invention is to avoid the disadvantages of conventional screening devices and to improve them in such a way that they are suitable for separating an egg cell-cumulus complex from a punctate, wherein the finding and the pipetting off of the egg-cumulus complex from the screening device to be relieved.
    According to the invention, this is achieved by a screening device which has a flat shell impermeable to punctate, and a cylindrical or conically widening wall region adjoining the edge of the shallow shell and having at least one sieve surface which is suitable for the egg cell-cumulus complex to be separated from the punctate. Furthermore, a preferably annular retaining edge is provided which limits a filling opening of the screening device.
    The bottom of the flat shell may be flat or slightly outwardly curved, is optically transparent or translucent, and smooth at least on the inner surface.
    The screening device is removed by the user from a sterile packaging. Then place the sieve in a sterile Petri dish and rinse the contents of up to ten collection tubes filled with punctate through the sieve. Thereafter, the sieve device is held with sterile tweezers on the retaining edge and discards the accumulated punctate in the Petri dish. Now you can rinse the screen with a prewarmed rinsing fluid and free of blood, put them back into the Petri dish and conveniently examine the transparent bottom of the sieve for the presence of OCC and umpettieren this in another shell. The remainder of the rinsing liquid remains in the flat shell of the screening device, which prevents drying out or sticking of the OCC and considerably simplifies pipetting up.
    The main advantage of the screening device according to the invention is thus that it can optimally seek out and remove the egg cell cumulus complex.
    A container for use of the screening device according to the invention is characterized in that the container is designed as a bottle with a gas-tight attachable lid, wherein in the lid an opening for gas-tight receiving a plug is formed, which plug a suction line of a Follikelpunktionssets and a connecting line to a vacuum source , wherein furthermore a holder for the screening device is provided on the inside of the cover, on the outlet side of the suction line.
    The invention will be explained in more detail below with reference to exemplary embodiments. Show it:
    1 shows a sieve device for separating an egg cell cumulus complex from a punctate in a three-dimensional representation,
    2 shows the screening device according to FIG. 1 in a sectional view, FIG.
    3 shows a container for use of a screening device according to FIG. 1 in a sectional representation, FIG.
    4 shows a variant embodiment of the container according to FIG. 3, and FIG. 5 shows a detail of the embodiment according to FIG. 4.
    Functionally identical parts are provided in the individual embodiments with the same reference numerals.
    The screening device 1 shown in Figs. 1 and 2 serves to separate an egg-cumulus complex from a punctate and has the following ranges: a flat shell 2 which is impermeable to punctate, one at the edge 4 of the flat shell 2 adjoining, cylindrical or conically widening wall region 5, which has at least one screen surface (6) which is suitable for separating the egg cell cumulus complex from the punctate and - a preferably annular retaining edge (8) delimiting a filling opening (7).
    The flat shell 2 can be realized either by a slightly outwardly curved bottom 3 (see dashed outline) or - as shown by solid lines - by a flat bottom 3 with a narrow collar-shaped edge 4. The remainder of the punctate or rinsing liquid remaining in the dish 2 ensures that the egg-cumulus complex does not dry out or adhere to the screening device and can therefore be picked up with a pipette without problems.
    Preferably, the cylindrical or flared wall portion 5 is divided into individual (e.g., four or six) screen surfaces 6 by a plurality of support ribs 9 extending between the edge 4 of the flat shell 2 and the retaining edge 8 bounding the filling aperture 7. The sieve surfaces 6 have a pore size between 60 pm and 100 pm, wherein the pore size is in any case smaller than the diameter of an egg cell.
    In order to be able to handle the sieve device 1 better with tweezers, the retaining edge 8 defining the filling opening 7 has a lateral grip tab 11. The retaining edge 8 together with grip tab 11 is arranged in a plane parallel to the bottom 3.
    The container 20 consists of a bottle 21 with a gas-tight or airtight attachable lid 22, preferably a screw cap, wherein in the lid 22 has an opening 23 for gas-tight or airtight recording of a plug 24 is formed. The stopper 24 encloses a suction line 25 of a follicle puncture set (see eg EP 1 967 147) and a connecting line 26 to a vacuum source (not shown, for example a vacuum pump), wherein on the inside of the lid, on the output side of the suction line 25, a holder 27 for the screening device 1 is provided is.
    According to the invention, the holder can be designed as a wire ring 28 which can be fastened to the lid 22 or the stopper 24 and on which the holding edge 8 of the sieve device 1 rests.
    For the preparation of the container according to Fig. 3, first an opening 23 is milled into the lid 22 in which the stopper 24 (e.g., silicone stopper) of a follicular puncture set is fixed. A sterile packed holder 27 (wire frame with wire ring 28) for inserting the screening device 1 is drilled in the bottom of the plug 24 and fixed thereto. Now a sterile packaged screening device 1 is placed with a sterile tweezers in the wire ring 28 of the wire frame and the bottle 21 with the lid 23 hermetically sealed.
    During the follicular puncture, the puncture flows through the suction line 25 into the screening device 1. Once the puncture in the bottle 21 (for example, a glass bottle or an at least partially transparent plastic material) has reached a certain height, this is discarded. With this technique, up to 15 follicles can be punctured in series.
    Thereafter, the bottle cap 22 is opened, removed the screening device 1 with a sterile forceps and placed in a sterile Petri dish. This is followed by the steps described above in connection with the sieve device for rinsing, searching and pipetting off the egg cell-cumulus complex.
    According to an embodiment variant shown in FIGS. 4 and 5, the holder 27 may be designed as a plastic part, which is attached to the inside of the lid 22 via connecting webs 29 or made in one piece with this. The holder 27 has a slot-shaped receptacle 30 for insertion of the retaining edge 8 of the screening device 1.
    In reproductive medicine, the motto is: the shorter the oocyte-cumulus complex is exposed to blood contact during follicular biopsy and the risk of cooling and dehydration, the better. The screening device according to the invention or the container for use of such a screening device are thus not only time-saving, but also represent a decisive advantage for the egg and its fertilization capacity.
    权利要求:
    Claims (10)
    [1]
    A sieve device (1) for separating an egg-cumulus complex from a punctate, characterized by a shallow dish (2) which is impermeable to the punctate, a cylindrical one adjacent to the edge (4) of the shallow dish (2) a conically widening wall region (5) which has at least one sieve surface (6) which is suitable for separating the egg cell cumulus complex from the punctate and a preferably annular retaining edge (8) delimiting a filling opening (7).
    [2]
    2. sieve device (1) according to claim 1, characterized in that the cylindrical or conically widening wall portion (5) by a plurality of support ribs (9) extending between the edge (4) of the flat shell (2) and the filling opening ( 7) limiting retaining edge (8), is divided into individual screen surfaces (6).
    [3]
    3. Screening device (1) according to claim 1 or 2, characterized in that the screen surfaces (6) have a pore size between 60 pm and 100 pm.
    [4]
    4. Screening device (1) according to one of claims 1 to 3, characterized in that the bottom (3) of the flat shell (2) is optically transparent or translucent and at least on the inner surface (10) smooth.
    [5]
    5. Screening device (1) according to one of claims 1 to 4, characterized in that the filling opening (7) limiting retaining edge (8) has a lateral grip tab (11).
    [6]
    6. container (20) for using a screening device (1) according to one of claims 1 to 5, characterized in that the container (20) as a bottle (21) with a gas-tight attachable cover (22), preferably a screw cap is executed wherein in the lid (22) an opening (23) for gas-tight receiving a plug (24) is formed, which stopper (24) has a suction line (25) of a Follikelpunktionssets and a connecting line (26) to a vacuum source and wherein on the inside cover On the output side of the suction line (25) a holder (27) for the screening device (1) is provided.
    [7]
    7. Container (20) according to claim 6, characterized in that the holder (27) as on the lid (22) or the plug (24) attachable wire ring (28) is executed, on which the retaining edge (8) of the screening device (1 ) rests.
    [8]
    8. Container (20) according to claim 6, characterized in that the holder (27) is designed as a plastic part which is attached to the inside of the lid (22) or manufactured in one piece with this.
    [9]
    9. Container (20) according to claim 8, characterized in that the holder (27) has a slot-shaped receptacle (30) for insertion of the retaining edge (8) of the screening device (1).
    [10]
    10. Container (20) according to any one of claims 6 to 9, characterized in that the bottle (21) is a glass bottle or an at least partially transparent plastic bottle.
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    同族专利:
    公开号 | 公开日
    AT519299B1|2018-11-15|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    DE3605384A1|1986-02-20|1987-08-27|Ludwig Bertram Gmbh|Insert in a container for the collection of an embryo-containing rinsing liquid|
    US4809860A|1987-12-07|1989-03-07|Mark L. Anderson|Aspiration container assembly for collecting follicular fluids|
    EP0593767B1|1991-07-08|1996-03-13|Nippon Becton Dickinson Company, Ltd.|Cell strainer|
    US9322748B1|2014-09-16|2016-04-26|II Melbourne Kimsey|Tissue collection and refining device and method|WO2021220309A1|2020-05-01|2021-11-04|Payeli Sravan Kumar|Oocyte separation|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    ATA51002/2016A|AT519299B1|2016-11-03|2016-11-03|SIEVE DEVICE AND CONTAINER FOR USING A SIEVE DEVICE|ATA51002/2016A| AT519299B1|2016-11-03|2016-11-03|SIEVE DEVICE AND CONTAINER FOR USING A SIEVE DEVICE|
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